The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or . objetivos: conocer las diferentes técnicas de aglutinación usadas en el laboratorio. adquirir habilidad y destrezas en las técnicas de. Prozone effect: No prozone effect was detected upon mg/dL. 4. . Efecto prozona: No se observa efecto prozona hasta valores de
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Term Bank – efecto prozona – Spanish English Dictionary
In this case, no sandwich can be formed by the capturing antibody, the antigen and the detection antibody. The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assaysresulting in false negatives or inaccurately low results.
However, when the serum is diluted, the blocking antibody is as well and its concentration decreases enough for the proper precipitation reaction to occur. The phenomenon is caused by very high concentrations of a particular analyte or antibody prozna is most prevalent in one-step sandwich immunoassays.
Retrieved 3 February Because no agglutination occurs, the test is interpreted as negative.
Thus the proper precipitation reaction does not take place. This page was last edited on 14 Augustat The Clinical Biochemist Efectto.
However, if too many antibodies are present that can bind to the antigen, then the antigenic sites are coated by antibodies, and few or no antibodies directed toward the pathogen are able to bind more than one antigenic particle. Retrieved from ” https: If the antibodies agglutinate with efect antigen to form immune complexesthen the test is interpreted as positive.
Views Read Edit View history. Examples include high levels of syphilis antibodies in HIV patients or high levels of cryptococcal antigen leading to false negative tests in undiluted samples.
Hook effect – Wikipedia
Has its time arrived? Other common forms of interference include antibody interference, cross-reactivity and signal interference.
The serological test is mainly seen in the precipitation reaction. In this case, free antigen is in competition with captured antigen for detection antibody binding. In an agglutination test, a person’s serum which contains antibodies is added to a test tubewhich contains a particular antigen. In this case, the result is a false negative.